Cucurbitacin B treatments in the parental MCF-7 and MDA-MB-231 (endogenously expressed wt-BRCA1), BRCA1 knocked-down counterparts and shRNA scrambled controls.

<p>(A) and (B), Western blot analysis for p21, p27 and survivin proteins after the cells were cultured either in control medium or in the medium containing 15 µg/ml cucurbitacin B for 48 h. GAPDH was used as loading control. (C), The degrees of significantly cytotoxic responses (indicating as percent killed) to paclitaxel and cucurbitacin B in wt-BRCA1 parental and BRCA1 knocked-down MCF-7 and MDA-MB-231 cells are shown, (* <i>p</i><0.01).