Effect of A549 cells and A549 cell-conditioned medium (CM) on septic PMN-dependent PMVEC permeability and trans-PMVEC PMN migration.

<p>Panels A,B: In cytomix-stimulated PMN/PMVEC co-cultures, PMVEC permeability and PMN migration were measured in the presence vs absence of A549 cells (non-contact co-culture model). Septic PMN-dependent PMVEC hyper-permeability and trans-PMVEC PMN migration were inhibited in the presence of A549 cells. Panels C,D: Similarly, A549-CM inhibited both septic PMN-dependent PMVEC hyper-permeability and trans-PMVEC PMN migration. *: P<0.05 and **: P<0.01 vs respective control; #: P<0.05 and ##: P<0.01 vs PMVEC alone (panels A,B) or EGM-treated (panels C,D).