NMDAR subunits immunofluorescence in mature hipocampal neuron cultures stimulated by KCl.

<p><b><i>A.</i></b> Quantification of NMDAR subunit puncta at dendrites (n = 100 neurites/culture). A significant increase in GluN1 and GluN2A puncta was observed at 30 and 70 minutes after KCl stimulation (** p<0.05, *** p<0.001, Kruskal-Wallis test followed by Dunn’s Multiple Comparison Post-Test). <i>Insert on the top of each bar</i>: representative dendrite for each condition (bar: 2 µm). <b><i>B.</i></b> Total fluorescence quantification 30 and 70 minutes after KCl stimulation. There were significant increases in GluN1 and GluN2A 70 minutes after stimulation. There were no significant changes in GluN2B total immunofluorescence (* p<0.05, *** p<0.001, ONE WAY ANOVA, Dunnet Post-Test). <i>Right:</i> representative neurons for each condition.