Several β-amyloid antibodies show high non-specificity for β-amyloid by western blot.

<p><b>A</b>) Western blot with the antibody M3.2 (specific for rodent β-amyloid) using hippocampal tissue from APP-KO, WT, and APP transgenic mice expressing human APP (Hu<i>APP</i>695SWE). All three genotypes show a similar band pattern. <b>B</b>) Western blot with the antibody 6E10 (specific for human β-amyloid). All three genotypes show a similar band pattern. However, with 6E10, the APP transgenic mouse tissue shows an additional 8 kD band (presumably a human β-amyloid dimer) as well as a band near 87 kD (presumably full-length human APP). In addition, a 4.5 kDa band (β-amyloid) is detected when older mouse brain is used that has high levels of β-amyloid protein. <b>C</b>) Western blot with 4G8. All three genotypes also showed a similar band pattern with this antibody as well. In addition, a 4.5 kDa band (β-amyloid) is detected when older mouse brain is used that has high levels of β-amyloid protein.