NMDAR subunits change after LTP induction and expression.

<p><b><i>A.</i></b> Evoked fEPSPs normalized slopes from fresh hippocampal slices corresponding to the first pulse of paired stimulation, before and after TBS (arrow). Plots represent the average of three independent experiments over 90 minutes of recording (n = 6 for each group). <i>Insert on top:</i> average traces of 10 individual recordings from a +TBS+LTP and a +TBS-LTP slices (black: 5 minutes before TBS; grey: 5 last minutes of recording). <b><i>B.</i></b> WB band densities quantification of samples from same slices that in <b>A.</b> A significant increase was only observed for +TBS+LTP slices (** p<0.01; *** p<0.001 ONE WAY ANOVA, Dunnet Post-Test; n = 6 for each group). <i>Insert on top:</i> (from left to right): representative GluN1 and GAPDH WB bands from: a −TBS slice, a +TBS-LTP slice and a +TBS+LTP slice. <b><i>C.</i></b> Evoked fEPSPs slopes corresponding to the first pulse of the paired stimulation before and after TBS (arrow). Plots represent the average of fEPSPs slopes over 50 and 90 minutes of recording, respectively (n = 6 for each group). <i>Right</i>: average traces of 10 individual recordings from a LTP-slice after 30 and 70 minutes TBS (black: 5 minutes before TBS; grey: 5 last minutes of recording). <b><i>D.</i></b> NMDAR subunits quantification by WB. Samples analyzed: slices used in <b>C</b>. (processed 30 or 70 minutes after TBS) and in −TBS slices (Control). Analysis of WB bands showed a significant increase in GluN1 and GluN2A level for the 70 minutes group in three independent experiments (* p<0,05; *** p<0,001 ONE WAY ANOVA-Dunnet Test). <i>Insert on top:</i> Representative WB bands for GluN1, GluN2A and GluN2B NMDAR subunits and GAPDH (internal control).