DMSO does not exacerbate neomycin-induced sensory hair cell death.

<p>Five dpf zebrafish larvae were treated with embryo medium (white bar), 100 µM neomycin sulfate (black bar), 0.5% DMSO (striped bar), or both for 1 hour (cross-hatched bar), and then washed three times in embryo medium. The larvae were allowed to recover in embryo medium for three hours as described previously <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055359#pone.0055359-Harris1" target="_blank">[25]</a>, and then fixed. Although the neomycin treatment resulted in significantly fewer sensory hair cells, co-treating the larvae with both DMSO and neomycin did not cause more hair cell death than neomycin alone. Results are the mean values ± SD. n = 10−12 neuromasts for each treatment group. *p<0.05 when individual treatments are compared to untreated controls.