Analysis of apoptosis.

<p>(A) FACS analysis of indicated subsets was performed by Annexin-V and 7AAD staining. The Annexin-V positive gate was set based on its intensity on 7AAD⁺ Lin⁻ cells, which represent Annexin-V positive dead cells. Numbers indicate the percentage in each quadrant. (B) Average percentages of Annexin-V⁺7AAD⁻ or Annexin-V⁻7AAD⁻ (viable) cells are shown with SD. Grey bar, untreated; black bar, treated with 1 µg of LPS for 30 days. *** p<0.0001.