MDS/EVI is strongly upregulated in the intestine at the climax of metamorphosis.

<p>A primer set common to EVI and MDS/EVI was used for qPCR analysis on total intestinal RNA at different stages during metamorphosis as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055585#pone-0055585-g002" target="_blank">Fig. 2</a>. Note that the data is consistent with that in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055585#pone-0055585-g002" target="_blank">Fig. 2</a> if one considers the fact that there is little non-Ep in premetamorphic tadpoles at stage 56 but non-Ep increases as a percentage of the total intestine during metamorphosis. Error bars indicate SEM (n = 3). * indicates transcript levels lower than the peak expression (p≤0.05).