Reduction in IL-4Rα expression correlates with magnitude of anti-viral effector responses on CD8⁺ T cells.

<p>Unimmunized or VV-WR infected BALB/c WT mice (n = 4 per group) were sacrificed at the indicated time points and splenocytes used for flow cytometry analysis or for ICS following A52_75–83 or F2_26–34<i>in vitro</i> peptide stimulation of splenocytes as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055788#s4" target="_blank">materials and methods</a>. A and B, The mean (n = 4) percentage of CD8⁺ splenocytes (A) and the total number of CD8⁺ splenocytes (B) from VV-WR infected mice that expressed GzmB or IFN-γ following <i>in vitro</i> peptide stimulation. C, The kinetics of the mean (n = 4) net fold reduction in cell surface expression of IL-4Rα on gated CD8⁺ splenocytes from VV-WR infected mice relative to unimmunized mice. Net fold reduction was calculated using MFI values as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0055788#s4" target="_blank">materials and methods</a>. The data shown are representative of at least two independent experiments and the error bars depict the SEM.