Detailed distribution of the PP-rich region in the head of the pancreas.

<p><b>A</b>: Virtual slice views of blocks in the head region. Immunostained for PP (red), insulin (green), glucagon (white) and nuclei (blue). All in the same scale. <b>B</b>: 3D visualization of islet size (area) and shape (circularity and Feret's diameter) distribution. Each dot represents a single islet/cluster. PP-cell containing clusters are shown in red. <b>C</b>: Quantitative analysis of individual islet size distribution and cellular composition. Relative frequency of islet size (gray bar) and ratios of beta (green), alpha (blue), and PP (red) cells within islets are plotted against islet size; means ± SEM.