Correctly identified fluorescent neurons by 2PM are proved to be neurons at the anastomosis in MOS-treated mice.

<p><i>A.</i> Green Fluorescent Protein (GFP)-positive cells. <i>B.</i> 5-bromo-2'-deoxyuridine (BrdU)-positive cells. <i>C.</i> A neural marker, neurofilament (NF)-positive cell. <i>D.</i> A neural stem cell marker, distal less homeobox 2 (DLX2)-positive cells. <i>E.</i> glial fibrillary acidic protein (GFAP)-negative cells. Red arrows indicate NF+/DLX2−/BrdU+/GFP+/GFAP- cell: this cell is a new neuron. Green arrows indicate NF−/DLX2+/BrdU+/GFP+/GFAP- cells: these cells seem to be neural progenitors. Similar results are obtained in other preparations.