IL-4, IL-13 and STAT6 regulate IL-4Rα expression on CD8⁺ T cells following VV-WR infection.

<p>Gene knockout mice (n = 5) and respective littermate WT control mice (n = 5) were infected with VV-WR for 7 days or kept unimmunized prior to sacrifice and analysis using flow cytometry. A, Representative histogram plots showing cell surface IL-4Rα expression on the indicated CD8⁺ splenocyte subset from a gene knockout mouse or a littermate WT control mouse. B, Mean (n = 5) MFI representing cell surface IL-4Rα expression on the indicated CD8⁺ splenocyte subset from mice of the indicated genetic background infected with VV-WR. C, Representative histogram plots showing cell surface IL-4Rα expression on the indicated CD8⁺ splenocyte subset from an unimmunized mouse or VV-WR infected mouse belonging to the indicated genetic background. All the results shown are representative of at least two independent experiments. Error bars when shown depict the SEM and one-way ANOVA (Tukey's Multiple Comparison) was used to determine the statistical significance of the data relative to WT control mice (*** - p<0.001).