Immunofluorescence detection of VEGF, VEGFRs and P-VEGFR-2 regulated by UVA in normal human epidermis.

<p>(a) Expression and localization of VEGF165, VEGFR-1, VEGFR-2, NRP-1 and P-VEGFR-2 by immunofluorescence regulated by UVA in normal human epidermis. (b) The fluorescence density analysis of (a). Skin samples from 5 independent individuals were used for quantification. Biopsies were taken 24 h after treatment of 0, one MED, and three MEDs of UVA respectively. The presence of VEGF165 and VEGFRs was indicated by red fluorescence. The presence of P-VEGFR-2 was indicated by green fluorescence. The cellular nuclei were counterstained with DAPI (blue nuclear signal). P-VEGFR-2, phospho-VEGFR-2 (Tyr1175); UVA: UVA; MED, minimal erythema dose; NC, negative controls, which were incubated with non-immune mouse IgG. Bars: 50 µm; Asterisk, epidermis; Yellow triangle, dermis; * <i>P</i><0.05; # <i>P</i><0.01.