Protein expression of Foxp3 in colon cancer cell lines by flow cytometry and immunofluorecence double staining analysis.
- Mia Kim (285988)
- Tanja Grimmig (285989)
- Martin Grimm (285990)
- Maria Lazariotou (285991)
- Eva Meier (285992)
- Andreas Rosenwald (321)
- Igor Tsaur (285993)
- Roman Blaheta (285994)
- Uwe Heemann (285995)
- Christoph-Thomas Germer (285996)
- Ana Maria Waaga-Gasser (285997)
- Martin Gasser (285998)
Publication date
February 2013
DOIAbstract
<p>(<b>A</b>) Flow cytometry assay of Foxp3 expression in SW480, SW620, and HCT-116 colon cancer cell lines compared to isotype control. 3.8% to 6.1% of colon cancer cells express Foxp3; PE: phycoerythrin; FS: forward scatter linear. (<b>B</b>) Representative examples of immunofluorescence double staining of Foxp3+ expression in SW480, SW620, and HCT-116 cancer cells. Cy3, indocarbocyanin red and DAPI 4′,6-Diamidino-2- phenylindoldihydrochlorid blue – nuclear counterstaining (×400 magnification).</p
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