AmplexRed peroxide determination by flow cytometry in HeLa and CHP-212.
- Alejandra Idan Valencia-Cruz (286040)
- Laura I. Uribe-Figueroa (286041)
- Rodrigo Galindo-Murillo (286042)
- Karol Baca-López (286043)
- Anllely G. Gutiérrez (286044)
- Adriana Vázquez-Aguirre (286046)
- Lena Ruiz-Azuara (286048)
- Enrique Hernández-Lemus (286050)
- Carmen Mejía (286052)
Publication date
February 2013
DOIAbstract
<p>The quantity of cells expressing Hydrogen peroxide was detected with AmplexRed by flow cytometry in the FL2-H channel, whereas SSCH corresponds to side scatter channel. Panel A: untreated HeLa cells, Panel B: Cas/HeLa, Panel C: untreated CHP-212 cells, panel D: Cas/CHP-212. Positive controls corresponds to 3 h UV-irradiated cells. Data is representative of three independent experiments for each experimental condition.</p
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