Runx2 gene and protein expression is detectable in neurons of the adult mouse brain.

<p>A) Qualitative PCR analysis of Runx2 mRNA (289 bp) expression in the suprachiasmatic nucleus (SCN) of adult mice. Lane 1: 100 bp molecular weight standard ladder (black arrowhead: 300 bp; grey arrowhead: 500 bp). Lane 2–4: Runx2 (289 bp) PCR product from reactions performed using E15 mouse calvarial bone mRNA extracts and Runx2-specific primers. Lane 5–7: Runx2 (289 bp) PCR product from reactions performed using adult mouse SCN mRNA extracts and Runx2-specific primers. Sequencing confirmed the identity of all positive PCR amplification products. B) Western blot analysis of Runx2 protein (∼60 kDa; black arrowhead) expression in the SCN of adult mice. Lane 1: Molecular weight standard ladder. Lane 2–3: protein isolated from E15 mouse calvarial bone. Lane 4–5: protein isolated from SCN of adult mice. GAPDH (∼37 kDa; grey arrowhead) protein detection served as a loading control. C) Immunolabelling of Runx2 (green) and NeuN (red) in the frontal cortex. Overlay image showing co-localization of Runx2 and NeuN (yellow). Scale bar: 50 µm.