PLD deletion results in abnormal phagosomal cup formation.

<p>Confocal microscopy of primary BMDM transfected via nucleofection with an EGFP-fused Spo20-PA sensor to image PA (green) in unstimulated WT BMDM (A) or in WT (B), <i>Pld1</i>^−/− (C), <i>Pld2</i>^−/− (D), or FIPI-treated WT (E) BMDM that were cultured with opsonized beads for 5 min. F-actin was visualized using rhodamine phalloidin (red). Scale bar, 5 µm. Best representative images from at least 3 sets of independently-isolated BMDMs. F) Circles shown indicating thickness of cup in WT BMDM superimposed on <i>Pld1</i>^−/− and <i>Pld2</i>^−/− cups, with quantitation of the thickness of the PA-visualized cups. At least 10 cups were measured for each experimental setting, and the experiments were performed 3 or more times. *** = p<0.0001.