Characterisation of TQS and 4BP-TQS in human induced pluripotent stem cell-derived neurons examined by fluorescence-based intracellular calcium imaging.

<p>A) Pre- and co-application of TQS (30 µM) with compound B (30 nM) potentiates the amplitude of compound B -evoked calcium-responses (almost undetectable in the absence of TQS) (Left). The trace represents the average response of 35 individual traces ± SEM. Responses to compound B (30 µM) co-applied with TQS (30 µM) are blocked when pre- and co-applied with MLA (1 µM; Right). The trace represents the average response of 137 individual traces ± SEM. B) Agonist-evoked calcium-responses were detectable in response to the allosteric agonist 4BP-TQS (30 µM; Left). The trace represents the average response of 122 individual traces ± SEM. Responses to 4BP-TQS (30 µM) were blocked by pre- and co-application of MLA (1 µM; Right). The trace represents the average response of 118 individual traces 5± SEM.