HSV-1-encoded gB signals through MyD88/TRAF6, but not TRAF2.

<p>HEK293T cells were transiently transfected with pCMV1-FLAG-hTLR2 and the NF-κB luciferase reporter plasmid along with vector (pCMV-HA or pCMV-FLAG) and various concentrations of pHA-DN-MyD88 (A), pDN-TRAF6 (B) or pFLAG-DN-TRAF2 (C). After 24–36 h, cells were stimulated with gBs (20 µg/ml) or zymosan (10 µg/ml) or left untreated for 8 h, and luciferase reporter gene activity was measured as described in <i>Materials and Methods</i>. Luciferase activity values are expressed as the ratio between firefly and <i>Renilla</i> luciferase. The results are shown as the mean ± SD of three independent experiments. Statistical analysis was performed using Student’s <i>t</i> test. (A) *, <i>P = </i>0.011. (B) *, <i>P = </i>0.023.