<i>syd-2</i> regulates the polarized distribution of neuropeptides in DB motorneurons.

<p>(A) Schematic diagram of a DB motor neuron with its axon in the dorsal nerve cord (DNC) and its dendrite in the ventral nerve cord (VNC)(top panel). This diagram is oriented with the anterior of the animal to the left for this and all subsequent figures. The boxed region denotes that the axon was imaged for data presented in panels (A–C, G–I). Representative images of INS-22::Venus in the axon of wild type, <i>syd-2(ok217)</i>, <i>syd-2(ju37)</i>, and <i>Punc-129::syd-2;syd-2(ju37)</i> rescue animals (bottom panels). (B–C) Quantification of INS-22::Venus puncta intensity (B) and density (C) in the axons of wild-type (n = 33), <i>syd-2(ok217)</i>(n = 24), <i>syd-2(ju37)(</i>n = 17), and <i>syd-2</i> rescue animals (n = 14). (D) Schematic diagram of DB motor neuron (top panel). The boxed region denotes that the dendrite was imaged for panels (D–F, J–L). Representative images of INS-22::Venus in the DB dendrite of wild-type, <i>syd-2(ok217), syd-2(ju37),</i> and <i>Punc-129::syd-2;syd-2(ju37)</i> rescue animals (bottom panel). (E–F) Quantification of INS-22::Venus puncta intensity (E) and density (F) in the dendrites of wild-type (n = 44), <i>syd-2(ok217)</i>(n = 31), <i>syd-2(ju37)</i>(n = 30), and <i>Punc-129::syd-2;syd-2(ju37)</i> rescue animals(n = 19). (G) Representative images of NLP-21::Venus in the DB axons of wild type and <i>syd-2(ju37)</i> mutant animals. (H-I) Quantification of NLP-21::Venus puncta intensity (H) and density (I) in the axons of wild type (n = 12) and <i>syd-2(ju37)</i>(n = 18) mutant animals. (J) Representative images of NLP-21::Venus in dendrites of wild type and <i>syd-2(ju37)</i> mutant animals. (K–L) Quantification of NLP-21::Venus puncta intensity (K) and density (L) in the dendrites of wild-type (n = 20) and <i>syd-2(ju37)</i>(n = 22) animals. For this and all subsequent figures, error bars denote standard error from the mean (SEM) and cell bodies in the images are marked by an asterisk. Values that differ significantly (Tukey-Kramer (B, C, E and F); Student’s <i>t</i> test (H, I,K, and L) from wild type (marked by asterisks above each bar) or from other genotypes (comparisons marked by brackets) are denoted on the graphs (#p≤0.05, *p≤0.01, **p≤0.001). (M) Schematic diagram of a DB neuron. The boxed region denotes that the cell body was imaged for data presented in panels (N-O). (N) Representative images of INS-22::Venus in the motor neuron cell bodies of wild-type and <i>syd-2(ok217)</i> mutant animals. The graph shows quantification of somatic INS-22::Venus puncta fluorescence intensity for wild-type (n = 24) and <i>syd-2(ok217)</i>(n = 22) mutant animals. (O) Representative images of NLP-21::Venus in the motor neuron cell bodies of wild type and <i>syd-2(ju37)</i> mutant animals. The graph shows quantification of somatic NLP-21::Venus puncta fluorescence intensity for wild type (n = 7) and <i>syd-2(ju37)</i>(n = 10) mutant animals. Values that differ significantly from wild type (Student’s <i>t</i> test) are denoted on the graphs (**p≤0.001).