Polycystin-2 expression in telomerase immortalized proximal tubule cells.

<p>Both telomerase immortalized normal kidney (N) proximal and PKD Q4004X (P) cells were grown to confluence for 3 or 7 days. Extracts were made with buffers containing non-ionic detergent and equivalent amounts of protein (50 µg) were loaded in each lane. Lanes labeled N and P represent lysates made from telomerase immortalized normal kidney proximal tubule and PKD Q4004X cells, respectively. Two bands at molecular weights 120 and 100 kDa are seen and the PKD cells have higher expression levels at both time points. At 7 days only the 100 kDa band is observed.