SYD-2 promotes the bi-directional movement of DCVs in DA3 axon commissures and dendrites.

<p>(A) Schematic diagram of a DA motor neuron (top panel). The boxed region denotes that the axon commissure was imaged for the data presented in panels (A–B). The graph (bottom panel) shows quantification of the average number of INS-22::Venus puncta moving anterogradely, retrogradely, or remaining stationary in kymographs generated from 20 s movies of mobile INS-22::Venus puncta in axon commissures of wild type (n = 29) and <i>syd-2(ju37)</i>(n = 18) mutant animals. (B) Quantification of the percentage of mobile and stationary INS-22::Venus puncta in the DA3 axon commissure of wild type (n = 325 puncta) and <i>syd-2(ju37)</i> (n = 141 puncta) mutant animals. (C) Schematic diagram of a DA motor neuron (top panel). The boxed region denotes that the dendrite was imaged for the data presented in panels (C-D). The graph (bottom panel) shows quantification of the average number of INS-22::Venus puncta moving anterogradely, retrogradely, or remaining stationary in kymographs generated from 20 s movies of mobile INS-22::Venus puncta in dendrites of wild type (n = 18) and <i>syd-2(ju37)</i>(n = 26) mutant animals. (D) Quantification of the percentage of mobile and stationary INS-22::Venus puncta in the DA3 dendrite of wild type (n = 257 puncta) and <i>syd-2(ju37)</i> (n = 405 puncta) mutant animals. Values that differ significantly (Student’s <i>t</i> test (A, C) and Chi Squared test (B, D)) from wild type are denoted on the graphs (# p≤0.05, *p≤0.01, **p≤0.001).