miR-221 induces cell proliferation in both SOSP-9607 and MG63 cell lines.

<p>Both SOSP-9607 and MG 63 cells were transfected with miR-221 mimic (221 Mi), miR-221 inhibitor (221 In) or scramble oligonucleotide (Scr). The non-transfected cells (blank) were used as control. After transfection, every 24 h, MTT assay was performed on four groups of SOSP-9607 (<b>A</b>) and MG 63 cells (<b>B</b>). The viable cell number was evaluated as the value of the absorbance at 490 nm with a reference wavelength of 630 nm. Values of optical density (OD) are expressed as means ± SD. Point symbol, mean of three independent experiments; bars, SD; *, p<0.05, **p<0.01. <b>C</b>, 48 h after transfection, cell cycle distribution of treated SOSP-9607 (<b>top</b>) and MG 63 (<b>bottom</b>) was measured using flow cytometry analysis.