Knockdown of endogenous FOXL2 down-regulates GnRH receptor (GnRHR) in immortalized human granulosa-luteal cells but not KGN cells.

<p>A, KGN cells were transfected with 50 nM control siRNA (si-Ctrl) or FOXL2 siRNA (si-FOXL2). RT-qPCR and Western blot were used to measure the mRNA (left panel) and protein (right panel) levels of FOXL2 and GnRHR. B, Immortalized human granulosa-luetal cells (hGL) were transfected with 50 nM control siRNA (si-Ctrl) or FOXL2 siRNA (si-FOXL2). RT-qPCR and Western blot were used to measure the mRNA (left panel) and protein (right panel) levels of FOXL2 and GnRHR. Results are expressed as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (p<0.05).