<i>Dlgh-1^−/−</i> mice display defects in skeletogenesis.

<p>Eviscerated carcasses of E18.5 <i>Dlgh-1^+/+</i>, <i>Dlgh-1^+/−</i>, and <i>Dlgh-1^−/−</i> mice on the FVB/NJ background were prepared and stained for cartilage and bone with alcian blue and alizarin red, respectively. Craniofacial skeleton in (A) <i>Dlgh-1^+/+,</i> (B) <i>Dlgh-1^+/−</i>, and (C) <i>Dlgh-1^−/−</i> mice. Arrows show loss of bone formation in <i>Dlgh-1^+/−</i> and <i>Dlgh-1^−/−</i> mice. F, frontal bone; IP, intraparietal bone; P, parietal bone; N, nasal; Ro, rostral bone; RTP, retrotemporal process; T, temporal bone. (D–G) Axial structures of <i>Dlgh-1^+/+</i> (D) and <i>Dlgh-1^−/−</i> mice (E). Forelimb (F) and hindlimb (G) structures. Arrows show the absence of bone formation in <i>Dlgh-1^−/−</i> mice. S, sternebrae; H, humerus; R, radius; U, ulna; F, femur; Fi, fibula; T, tibia. Scale bar in A-E = 1000 µm, in F = 650 µm and in G = 500 µm.