Elevation of Snord3aA expression in Tg MHu2M E199K mice brains is disease dependent.

<p>Total RNA extracted from brains of TgMHu2ME199K (tg), PrP ^0/0 (ko) and wt mice, od designated ages were reverse transcribed into cDNA and amplified by Real Time PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054433#s4" target="_blank">Materials and methods</a>. Each group comprised 4–7 mice. Gene expression changes are depicted in relevance to those measured at 3 months old wt mice. Relative expression levels were normalized in reference to UBC. (** P value <0.001).