The effect of Gö6976, PD98059 and sulodexide on fibronectin and collagen type III synthesis and phosphorylation of signaling pathways in murine mesangial cells.

<p>(A) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence Gö6976 or PD98059 on fibronectin and collagen type III synthesis in murine mesangial cells after 24 h incubation (upper panel). The intensity of the bands were analyzed by densitometric scan using ImageJ (NIH), normalized to β-actin and expressed as arbitrary densitometric units (DU) (lower panels). ***<i>P</i><0.001, 5 mM D-glucose vs 30 mM D-glucose, ^###<i>P</i><0.001, 30 mM D-glucose vs 30 mM mannitol, ^§§<i>P</i><0.01 or ^§§§<i>P</i><0.001, with vs without inhibitor for the same stimulation. (B) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence of sulodexide on fibronectin and collagen type III synthesis in murine mesangial cells after 24 h incubation. (C) Western blot analysis showing the effect of 5 mM D-glucose, 30 mM D-glucose and 30 mM mannitol in the presence or absence of sulodexide on ERK PKC-α, PKC-βI or PKC-βII phosphorylation in murine mesangial cells after 24 h incubation.