Expression of Wnt5a gene in HBE cells and normal matched lung tissues

<p>. (A) mRNA and protein levels of Wnt5a expression were evaluated by RT-PCR (left), quantitative RT-PCR (middle) and Western blot analysis (right) in lung carcinogenesis in five HBE cells (NHBE, BEAS-2B, 1179, 1198 and 1170I) with different cell phenotype by exposure to cigarette smoke condensates. (B) mRNA levels of Wnt5a in 12 normal matched lung tumor tissues (7 smokers and 5 non-smokers) by RT-PCR. N: normal tissue, T: tumor tissue. Quantification of data means the average intensity of Wnt5a bands normalized by GAPDH. Bars denote standard error. Significant differences (*: p<0.03) from normal tissues as determined by a Student's t test.