pSTAT3 and c-fos expression in ARC neurons in leptin-treated mice.

<p>A–D) Representative immunohistochemistry (IHC) images showing pSTAT3 immunoreactivity following ip PBS (saline) or leptin injection. Scale bar, 100 µm. E) <i>Magel2</i>-null mice have fewer pSTAT3 positive cells than control following leptin injection (Bregma −1.28, <i>P</i><0.001; Bregma −1.64, <i>P</i><0.005; Bregma −2.12, <i>P</i>>0.5, *compared between genotypes by Student's <i>t</i>-test) but both genotypes have more pSTAT3 positive cells after leptin treatment than after PBS (#, <i>P</i><0.05, compared between treatments by Student's <i>t</i>-test; <i>n</i> = 8 mice of each genotype). F–I) Representative images of c-fos IHC following ip PBS and leptin injection. Scale bar 100 µm. J) Leptin induced c-fos expression in both <i>Magel2</i>-null and control mice compared to PBS (#<i>P</i><0.05, compared between treatments by Student's <i>t</i>-test). At both baseline and following leptin treatment, <i>Magel2</i>-null mice had significantly fewer c-fos positive cells compared to controls (Bregma −1.28 ∼18% reduction, Bregma −1.64 ∼20% reduction, Bregma −2.12 ∼35% reduction, *<i>P</i><0.05, compared between genotypes by Student's <i>t</i>-test). Values in E) and J) are means ± SEM.