The involvement of Pol II in milRNA production.

<p>A. Race tube assays showing the growth rates of the indicated strains in the presence or absence of QA. B. Northern blot analysis showing the levels of <i>rpb5</i>-specific siRNA in the indicated strains. C and D. qRT-PCR analysis results showing the reduction of <i>rpb5 and β-tubulin</i> level in ds<i>rpb5</i> strain in the presence of QA. The asterisks indicate <i>P</i> value<0.05. Error bars indicate S.D. E. Northern blot analysis showing the levels of <i>milR-1</i> and <i>milR-4</i> small RNAs. F. ChIP assay using c-Myc antibody showing the binding of Pol II to milR loci in the Myc-RPB6 strain. A <i>tRNA</i> and the <i>β-tubulin</i> gene was served as the negative control and positive control, respectively.