Effect of endoglin haploinsufficiency on TGFβ1 signaling pathways.

<p>(<b>A</b>) <i>Eng^+/+</i> and <i>Eng^+/−</i> MDF were stimulated with 1 ng/ml TGFβ1 for 30 minutes. Total protein extracts were analyzed by western blot with anti-phospho-Smad1/3, anti-phospho-Smad2, anti-Smad1 and anti-Smad2/3 (<b>B</b>). Id1 expression was assessed by western blot after 1 hour TGFβ1 treatment. (<b>C</b>) TGFβ1-induced PAI-1 expression increment was analyzed by quantitative RT-PCR. A representative experiment of three independent experiments using triplicate samples is shown. (<b>D</b>–<b>F</b>) MAPKs and PI3K/Akt pathways were analyzed after stimulation with 1 ng/ml TGFβ1 for 5, 15 and 30 minutes. Total proteins extracts were resolved by western blot and incubated with anti-phospho-Erk1/2 and anti-Erk1/2 (<b>D</b>), anti-phospho-JNK1 and anti-JNK1 (<b>E</b>) and anti-phospho-Akt and anti-Akt (<b>F</b>) respectively. Tubulin was used as loading control. Mean+SEM is represented (n = 3). * p<0.05-significance of the difference between TGFβ1 treatment and control conditions, Two-way ANOVA. A representative blot from three independent experiments is shown.