Induction of oxidative stress in kidneys of rats postburn.

<p>Burn injury induced sustained ROS generation evidenced by increased renal (<b>A</b>) H₂O₂ production, (<b>B</b>) thiobarbituric acid-reactive substances (TBARS) generation, suppressed antioxidant activity evidenced by decreased (<b>C</b>) SOD level and at 3 h, 6 h, 24 h, 48 h and 72 h after burn, which were attenuated by tempol at 72 h after burn. Renal (<b>D</b>) MnSOD activity and (<b>E</b>) protein expression, and (<b>F</b>) Nox4 expression was analyzed to determine the mitochondrial status. Treatment with tBHP also induced significant oxidative stress in kidneys of sham rats. *<i>P</i><0.05, **<i>P</i><0.01 vs. Sham; ^##<i>P</i><0.01 vs. Vehicle (72 h). n = 6 rats per group per time point.