Effect of PI3K inhibition on endoglin-mediated different cell proliferation.

<p>(<b>A</b>) Cell number of MDF was analyzed by crystal violet assay after 10 μM LY294002 treatment for 4 days. Inhibition of Akt activation after LY294002 treatment was analyzed by western blot (<b>A</b>, upper panel). (<b>B</b>) BrdU incorporation of <i>Eng^+/+</i> and <i>Eng^+/−</i> MDF after LY294002 treatment was also analyzed. (<b>C</b>) Endo and Mock fibroblasts proliferation was assessed by MTT after 10 μM LY294002 treatment. Inhibition of Akt activation after LY294002 treatment was analyzed by western blot (<b>C</b>, upper panel). Mean+SEM is represented (n = 3). * p<0.05-significance of the difference between cells in control conditions, ** p<0.05-significance of the difference between LY294002 treatment and control conditions, Two-way ANOVA. Tubulin was used as loading control. A representative blot from three independent experiments is shown.