Mitochondrial morphology is abnormal in anxA6 null cells.

<p>(A) Images of primary fibroblasts from <i>AnxA6</i>^−/− mice immunostained for cytochrome c and analysed by confocal microscopy to visualise mitochondria. (B) Mitochondrial morphology was graded as tubular, intermediate or fragmented for <i>AnxA6</i>^−/− and control mice. Data represent the mean ± s.d. of 3 independent experiments, with >1000 cells counted for each strain. (C) A431 cells were either labelled in vivo with mitochondrial-targeted GFP (mtGFP), or labelled following fixation with antibodies to Cyto-c. Cells were counter-stained using DAPI and visualised by confocal microscopy. Representative images show that mitochondrial staining is fragmented in control A431 cells, but tubulated in A431 cells stably expressing AnxA6. Scale bar = 2 µm. The western blot shows expression of the larger isoform of anxA6 only in the stable transfected cells.