Partial co-localisation of AnxA6 to mitochondria.

<p>(A) Mitochondria (M) and cytosol (C) were purified from normal mouse liver and western blotted for AnxA6 and AnxA2. The mitochondrial samples were immunoblotted following repeated washes (lanes w1-w4 show protein in each wash) in buffer containing 1 mM EGTA, as were whole liver cell extracts from control (WT) and <i>AnxA6</i>^−/− (KO) mice. (B) Normal primary mouse fibroblasts were co-immunostained for AnxA6 and cytochrome C and analysed by confocal microscopy. Insets show partial co-localisation of AnxA6 and Cyto-c on mitochondria. (C) Primary mouse fibroblasts were isolated from the ears of <i>AnxA6</i>^−/− mice and fixed and immunostained for AnxA6 and Cyto-c The images show faint non-specific staining with the AnxA6 antibody, and the characteristic pattern of fragmented mitochondria (see inset). Scale bar = 4 µm.