Sox2 is expressed in the majority of normal basal-epithelial cells, and cultures derived from prostate epithelial cells (PrECs) are either uniformly Sox2-Positive or Negative.

<p><b>A</b>) Immunofluorescent co-staining of Sox2 (green) with the basal-specific marker p63 (red), showing that 75% of normal basal-epithelial cells are positive for both Sox2 and p63 (yellow) while the remaining 25% are Sox2-negative (red). DAPI staining highlights nuclei (representative images of normal prostate epithelium acquired from three different individual patient specimens). <b>B</b>) Western blotting of a series of patient-derived Prostate (PrEC) and Seminal Vesicle (SVEC) epithelial sell (PrEC) cultures demonstrates that a portion of these cultures express detectable Sox2, while other PrEC and all SVEC cultures do not. <b>C</b>) Immunocytochemical staining of Sox2 showing uniform nuclear Sox2 expression in Sox2-positive PrECs and lack of Sox2-positive cells within Sox2-negative PrECs (representative images of three independent experiments).