Impact of diet on sperm functionality: sperm capacitation and acrosome reaction.

<p>Protein tyrosine phosphorylation (A) and acrosomal exocytosis index (B) of rabbit spermatozoa. A: phospho-Y proteins from control (NCR), HCR and ½ HCR+½ OO showed different patterns ranging from one band (non-capacitated, culture medium without BSA (−), approximately 60 kDa) to many bands (capacitated with BSA (+), from over 20 to 100 kDa). Notice that capacitated sperms from HCR decreased the p-Y protein pattern compared with NCR, and ½ HCR+½ OO shows a p-Y pattern resembling control (NCR) conditions. The experiment was performed at least three times and representative blot is shown. B: Bars represent AR index of spermatozoa from NCR, ½ HCR, ½ HCR+½ OO and OO after 10 µM progesterone incubation. AR index corresponds to normalized data (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0052386#s2" target="_blank">Materials and Methods</a>). n = 25 samples. *** = significantly different from NCR (p<0.001).