Expression analysis of key transcription factors.

<p>Immunohistochemistry was performed in control (<b>A</b>, <b>C</b>, <b>E</b>, <b>G</b>, <b>I, K</b>) and CPTD6 (<b>B</b>, <b>D</b>, <b>F</b>, <b>H</b>, <b>J, L</b>) mice. (<b>A</b>, <b>B</b>) Nuclear expression of the pancreatic islet marker Islet 1 (Isl1) is retained in CPTD6 mice, showing that the islet cells are present. (<b>C,D</b>) Expression of Nkx2.2 is also unaffected in CPTD6 mice. (<b>E, F</b>) In contrast expression of Nkx6.1 is reduced in Pax6 deficient cells. (<b>G, H</b>) The nuclear expression of the transcription factor Pdx1 is lost in CPTD6 mice. Images are representative of at least four animals from each group. Scale bars, 50 µm.