Restricting low temperature frequency output constraints high temperature variability.

<p>Three models were generated with the same reference parameters, but different values. Temperature dependence fixed at: = = 1.5, = 1.6, = 3. Frequency, amplitude and duty cycle maps were generated (as in <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002857#pcbi-1002857-g005" target="_blank">Figure 5</a>) for the three parameter points, varying temperature and inward conductance: (A) = 0.1 µS, (B) = 0.075 µS, and (C) = 0.06 µS. The white boxes constrain a region from 10 to 11°C where frequency is between .95 and 1.05 Hz. The horizontal lines represent the vertical boundaries of the box and are plotted explicitly below each map to demonstrate the high temperature variability. Line 1 – = 0.0696 µS; line 2 – = 0.0645 µS; line 3 – = 0.0639 µS; line 4 – = 0.0563 µS; line 5 – = 0.0587 µS; line 6 – = 0.0486 µS. White mark corresponds to bifurcation coalescence point that defines the parameter region for <a href="http://www.ploscompbiol.org/article/info:doi/10.1371/journal.pcbi.1002857#pcbi-1002857-g008" target="_blank">Figure 8</a>.