6h sleep deprivation causes iNOS-dependent increases in basal forebrain adenosine release in mice.

<p>Comparison of adenosine release and tone following 6 h sleep deprivation in mice either with or without 1400 W. (a) AMPA-evoked release was significantly higher in slices after 6 h SD (n = 15) than control (n = 10), but was not significantly greater than 6 h SD incubated with 1400 W. (b) Basal tone however was significantly greater in 6 h SD mice than when incubated with 1400 W and also controls (b). Asterisks indicate significant difference (Mann-Whitney test, p<0.05). (c) Raw data traces from representative experiments for control (black) 6 h SD (light grey) and 6 h SD +1400 W (dark grey) normalised to 10 µM ADO’ calibration for tone measurements are shown (c). Bold arrows indicate the point of sensor removal from the slice causing artefacts, and those on the right tone measured by difference before and after removal.