Sublethal PTPMT1 knockdown sensitizes cells to the chemotherapeutic paclitaxel.

<p>(A–C) HeLa cells were transfected with 5 nM non-targeting siRNA (A), PTPMT1 siRNA#1 (B), or PTPMT1 siRNA#2 (C) for 30 hours before treatment with a dose response of sublethal paclitaxel (up to 5 nM) for 24 hours. Viability was measured using Cell Titer Glo. (D) Viability of untreated HeLa cells transfected with each siRNA at 54 hours. (E, F) Quantification of HeLa cell viability (E) or HeLa cell death by propidium iodide exclusion (F) with 5 nM non-targeting or PTPMT1 siRNA (54 hr total treatment) and 5 nM paclitaxel treatment (24 hr total treatment). For each experiment, error bars indicate standard deviation of three experiments. Statistical significance was calculated using a student’s t test; * - p<0.05; ** - p<0.01; *** - p<0.001.