Enhanced susceptibility to <i>SSITL</i> silenced transformant A10 induced by the transient expression of <i>SSITL</i> in the host plants.

<p>(A, B) The lesions induced by silenced transformant A10 and the wild-type strain Ep-1PNA367 on the leaves of tobacco (<i>Nicotiana benthamiana</i>) for 48 hr at 20°C. (C, D) The lesions induced by silenced transformant A10 and the wild-type strain Ep-1PNA367 on the leaves of <i>A. thaliana</i> Col-0 for 48 hr and 24 hr at 20°C, respectively. <i>SSITL</i> was expressed transiently in plants leaves by infiltrating with Agrobacterium GV3101 strain carrying <i>SSITL</i> expression vector. The leaves infiltrated with GV3101 carrying empty vector were selected as control (CK). Asterisks indicate statistical differences from the control (<i>P</i><0.05). (E, F) Transcript levels of <i>PDF1.2</i> and <i>PR-1</i> in the <i>SSITL</i> transiently expressed <i>A. thaliana</i> leaves (black) after being respectively inoculated with silenced transformant A10 and the wild-type strain Ep-1PNA367 for 3, 6, 9 and 12 hr, with the plant leaves infiltrated with the GV3101 carrying empty vector being sampled for control (red). (G) Analysis the <i>SSITL</i> expression in the transiently expressed leaves of <i>A. thaliana</i> and tobacco (<i>N. benthamiana</i>) with RT-PCR. The leaves infiltrated with the GV3101 carrying empty vector were selected as control (CK). The <i>A. thaliana GAPDH</i> and <i>N. benthamiana actin</i> genes (see primers in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053901#pone-0053901-t001" target="_blank">Table 1</a>) were used to normalize different samples.