The effect of EPO on the expression of inflammatory signaling events in the liver.

<p>The levels of activated NF-<i>κ</i>B p65 in the liver were determined by DNA binding-based ELISA (<b>A</b>). The relative levels of TLR4 expression were characterized by RT-PCR (<b>B</b>) and Western blot assays (<b>C</b>). The relative levels of JNK (<b>D</b>), ERK (<b>E</b>) and p38 MAPK (<b>F</b>) phosphorylation in the liver were characterized by Western blot assay and densimetric analysis. Data are representative images or expressed as the mean ± SEM of each group (n = 6) of mice from three separate experiments. ^#P<0.05 vs. the NC group, *P<0.05 vs. the HFD-Con group.