DIM blocked the fibrosis induced by pressure overload.

<p>(A) Representative images of PSR staining form indicated groups. (B) Quantitative analysis of left ventricle interstitial collagen volume fraction in indicated groups (n = 6). (C) Real-time PCR analysis of the mRNA expression of TGF-β1, TGF-β2, collagen Iα, col1agen β and CTGF in the myocardium obtained from indicated groups (n = 6). (D) Representative images of PSR staining form indicated groups. (E) Quantitative analysis of left ventricle interstitial collagen volume fraction in indicated groups in reverse experiments (n = 6). (F) Real-time PCR analysis of the mRNA expression of TGF-β1, TGF-β2, collagen Iα, col1agen β and CTGF in the myocardium obtained from indicated groups in reverse experiments (n = 6). The results were reproducible in three separate experiments. Values are expressed as mean±SEM. *<i>P<</i>0.05 compared with the corresponding sham group. ^#<i>P<</i>0.05 <i>vs</i> Vehicle+AB group.