Specificity of the anti-MAP polyclonal antibodies contained in the rabbit antiserum measured by western blot analysis.

<p>The total protein extracted from the HCC97-H cells was used to react with the anti-MAP polyclonal antibodies. The commercialized polyclonal rabbit anti-human HPSE antibody was used as positive control and the corresponding rabbit pre-immunized serum was used as negative control. A very clear band of about 50 kDa and a clear band of about 65 kDa are recognized in column 1, using the commercialized heparanase antibody (1∶200). Both 65 kDa and 50 kDa bands are exhibited in column 2, using the anti-MAP antiserum (1∶3000). No band is detected in the corresponding locations in column 3, using unimmunized rabbit serum (1∶3000).