Cry34Ab1/Cry35Ab1 competition binding with Cry proteins.

<p>(<b>A</b>) Competition binding between ¹²⁵I-Cry35Ab1 in the absence of Cry34Ab1 and unlabeled Cry35Ab1, Cry3Aa, or Cry6Aa. (<b>B</b>) Competition binding between ¹²⁵I-Cry35Ab1 in the presence of 250 nM Cry34Ab1 and unlabeled Cry35Ab1, Cry3Aa, Cry6Aa or Cry8Ba. The data for homologous competition between labeled and unlabeled Cry35Ab1 in panel B were an average of 9 replicates (3 replicates for 3 independent experiments); all other data were the average of triplicate assays. The concentration of western corn rootworm BBMV was 0.1 mg/ml. The concentration of ¹²⁵I-Cry protein was 5 nM, and the increasing concentrations of the unlabeled competitors were indicated. Error bars represent standard deviation.