Total cytosolic RNA was prepared used for Q-PCR analysis of the expression of IL-1β.

<p>Data are expressed as fold changes vs each paired unstimulated control, taken as calibrator for comparative quantitation analysis of mRNA levels. Each sample was measured in triplicate. (<b>A</b>) Data are the means ± SEM (n = 10). (<b>B</b>) Individual data of patients. FU = Follow up.