Developmental series of larvae images, including polarized microscopy.

<p>A–C) Fifteen day old prism larvae imaged under brightfield and polarized light. Aberrant skeletal and morphological development is found in the 510 µatm (B) and 730 µatm (C) treatments. D–F) Twenty day old 4-arm plutei in three treatments as imaged for morphometric measurements. G–I) Thirty day old plutei. Spicule nuclei for the developing posterodorsal arms are visible in the polarized light image of control treatment larvae (G-arrow). Smaller larvae with short arms are visible in the 510 µatm and 730 µatm treatments (H, I).