Effect of miR-138 on NGAL expression and cell migration.

<p>(A) Images of cell migration of different cell lines. Using the wound-healing assay for the three cancer cell lines, wounds were compared at 0 and 24 h after scratching. Light microscope image at 100×magnifications. (B) Western blot for NGAL expression in miR-138-transfected AsPC-1 cells. (C) For the transwell cell migration assay, cells that had migrated after a specified period were quantified using crystal violet staining. (D) The effect of miR-138 on (a) NGAL expression as determined by western blot analysis, and (b) cell migration for RL95-2 cells. ** indicates <i>p</i><0.01, *** indicates <i>p</i><0.001. NC: negative control, precursor miRNA.