Luteolin inhibited the activation of VEGFR2-mediated signaling pathways.

<p>(a) Luteolin suppressed the activation of VEGFR2 and their downstream signaling pathway triggered by VEGF in HUVECs. Proteins from different treatments was tested by Western blotting and probed with specific antibodies. Experiments were repeated for three times. (b) Luteolin inhibits the MMP-2 and MMP-9 production by HUVECs. 1) Condition medium from untreated HUVECs without trypsin activation. 2) Condition medium from untreated HUVECs after trypsin activation. 3) Condition medium from untreated HUVECs after trypsin activation+EDTA. 4) Condition medium from pretreated HUVECs (40 µmol/L luteolin) after trypsin activation. 5) Condition medium from pretreated HUVECs (20 µmol/L luteolin) after trypsin activation.